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Isolation and characterization of polymorphic microsatellites in the Tibetan cypress Cupressus gigantea using paired-end Illumina shotgun sequencing

  • 【作者】Shaoke Li ,Zengqiang Qian ,Yaru Fu ,Weilie Zheng ,Huie Li
  • 【DOI】10.1007/s12686-014-0222-8
  • 【作者单位】1. Institute of Plateau Ecology, Tibet Agricultural and Animal Husbandry College, Nyingchi, 860000, People’s Republic of China 2. Key Laboratory of Forest Ecology in Tibet Plateau (Tibet University), Ministry of Education, Nyingchi, 860000, Tibet, People’s Republic of China 3. College of Life Sciences, Shaanxi Normal University, Xi’an, 710119, Shaanxi Province, People’s Republic of China 4. Department of Food Science, Tibet Agricultural and Animal Husbandry College, Nyingchi, 860000, Tibet, People’s Republic of China
  • 【年份】2014
  • 【卷号】Vol.6 No.3
  • 【页码】795-797
  • 【ISSN】1877-7252
  • 【关键词】Cupressus gigantea Endangered species Microsatellites Next-generation sequencing 
  • 【摘要】 Cupressus gigantea Cheng et L. K. Fu is an endangered conifer endemic to Tibet of China. In this study, 16 polymorphic microsatellite markers were developed using paired-end Illumina shotgun sequencing. The number of alleles varied between 3 and 18, ...
  • 【文献类型】 期刊
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