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One-Pot Isothermal LAMP-CRISPR-Based Assay for Klebsiella pneumoniae Detection

  • 【获取途径】 OA资源
  • 【作者】Xiaotong Qiu, Xueping Liu, Xiao Ma, Ruixue Wang, Shenglin Chen, Fang Li, Zhenjun Li
  • 【作者单位】Affiliations 1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. 2 School of Laboratory Medicine and Life Sciences, Wenzhou Medical Universitygrid.268099.c, Wenzhou, China. 3 Department of Clinical Laboratory, Beijing Friendship Hospitalgrid.411610.3, Capital Medical University, Beijing, China. 4 Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, China. 5 School of Public Health, Shanxi Medical University, Taiyuan, China. 6 Department of Medicine, Tibet University, Lhasa, China.
  • 【年份】2022
  • 【页码】e0154522
  • 【ISSN】2165-0497
  • 【关键词】CRISPR CRISPR-top Cas12b Klebsiella pneumoniae LAMP accurate diagnosis nucleic acid detection. 
  • 【摘要】 Klebsiella pneumoniae is one of the most common pathogens causing nosocomial infection. A rapid, accurate, and convenient detection method is required for early diagnosis and directed therapy of K. pneumoniae infection. CRISPR-top is a LAMP-CRISPR-...
  • 【文献类型】 期刊
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