【作者单位】1 Laboratory of Natural Products and Metabolic Engineering, Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Sciences, Southwest University, Chongqing 400715, People’s Republic of China.; 2 Department of Biology, Chongqing University of Science and Technology, Chongqing 401331, People’s Republic of China.; 3 College of Pharmaceutical Science, Southwest University, Chongqing 400715, People’s Republic of China.; 4 Agricultural and Animal Husbandry College, Tibet University, Linzhi of Tibet 860000, People’s Republic of China.
【年份】2009
【卷号】Vol.3 No.5
【关键词】MECPS gene cloning color complementation assay Taxus media expression.
【摘要】 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MECPS, EC: 4.6.1.12) is the fifth enzyme of the methylerythritol phosphate (MEP) pathway of Taxol biosynthesis. The full-length cDNA sequence (designated as TmMECPS) was cloned and characterized ...
